~~NOTOC~~ ===== e2boxer_old ===== Particle Coordinates: Generate files containing particle coordinates for all input micrographs by picking particles manual and/or automatically. \\ ===== Usage ===== Usage in command line e2boxer_old.py input_micrograph_list --boxsize=BOXSIZE --write_dbbox --write_ptcls --exclude_edges --force --format=FORMAT --norm=NORM --invert --suffix=SUFFIX --dbls=DBLS --autoboxer=AUTOBOXER --ppid=PPID --gui --verbose=n --gauss_autoboxer=GAUSS_AUTOBOXER --do_ctf=DO_CTF --cter --indir=INDIR --nameroot=NAMEROOT --micsuffix=MICSUFFIX --wn=WN --Cs=CS --voltage=VOLTAGE --ac=AC --kboot=KBOOT --debug --apix=APIX \\ ===== Typical usage ===== e2boxer_old exists in non-MPI version. \\ __Start the e2boxer_old GUI with a list of micrographs__: e2boxer_old.py micrographs_number* \\ ===== Input ===== === Main Parameters === ; input_micrograph_list : Input micrographs: Wild cards (e.g. *) can be used to specify a list of micrographs. Not recommended if their number is very large. (default required string) ; %%--%%boxsize : Box size [Pixels]: Box size for extraction of particle images. (default -1) \\ === Advanced Parameters === ; %%--%%write_dbbox : Write coordinate files: Write coordinate files. (default False) ; %%--%%write_ptcls : Write particle images: Write particle images. (default False) ; %%--%%exclude_edges : Exclude edge images: Exclude particles extending outside the micrograph. (default False) ; %%--%%force : Force overwrite: Force overwrite. (default False) ; %%--%%format : Particle image format: Format of the output particles images. (default hdf) ; %%--%%norm : Particle normalization: Normalization processor applied to the final particle images. (default normalize.edgemean) ; %%--%%invert : Invert contrast: Invert contrast of micrographs. (default False) ; %%--%%suffix : Particle name suffix: Suffix used for the name of the output images and coordinates. (default _ptcls) ; %%--%%dbls : Data base list storage: Data base list storage, used by the workflow. (default none) ; %%--%%autoboxer : Swarm_boxers dict key: A key of the swarm_boxers dict in the local directory, used by the workflow. (default none) ; %%--%%ppid : Set PPID: Sets the PPID of the process. (default -1) ; %%--%%gui : Dummy option: Dummy option used in even older version of e2boxer_old. (default True) ; %%--%%verbose : Verbose: Verbose level. Accepted values 0-9. (default 0) ; %%--%%gauss_autoboxer : Sample autoboxed image: Gets the parameters used for autoboxing from the EMANDB. It requires the name of a micrograph used for automatic boxing previously. (default none) ; %%--%%do_ctf : CTF parameters file: Specify name of file whose CTF estimation parameters should be used for automatic CTF estimation. (default none) ; %%--%%cter : Use CTER CTF estimation: CTF estimation using CTER. (default False) ; %%--%%indir : Input directory: Directory containing micrographs to be processed. (default current directory) ; %%--%%nameroot : Micrograph rootname: Rootname of micrographs to be processed. (default none) ; %%--%%micsuffix : Micrograph type: A string denoting micrograph type. (default none) ; %%--%%wn : Window size: size of window to use. (default 256) ; %%--%%Cs : Spherical aberration [mm]: Spherical aberration [mm] used to acquire the input micrographs. (default 2.0) ; %%--%%voltage : Microscope voltage [kV]: Microscope voltage [kV] used to acquire the input micrographs. (default 300.0) ; %%--%%ac : Amplitude contrast: Expected amplitude contrast of this dataset. (default 10.0) ; %%--%%kboot : kboot: The number of boot-strap trials. (default 16) ; %%--%%debug : Debug mode: Use debug mode. (default False) ; %%--%%apix : Pixel size [A]: Pixel size of the input micrographs. (default -1.0) \\ ===== Output ===== The program creates the directory automatically in the micrograph directory. \\ ===== Description ===== \\ ==== Method ==== \\ ==== Reference ==== \\ ==== Developer Notes ==== \\ ==== Author / Maintainer ==== EMAN2 Group \\ ==== Keywords ==== Category 1:: APPLICATIONS \\ ==== Files ==== programs/e2boxer_old.py \\ ==== See also ==== [[http://blake.bcm.edu/emanwiki/EMAN2/Programs/e2boxer|e2boxer]] and [[http://blake.grid.bcm.edu/emanwiki/EMAN2/boxerFAQ|e2boxer FAQ]] \\ ==== Bugs ==== There are no known bugs so far. \\